https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2018Palovcak_Graphene 2026-05-24T20:17:59Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2018Palovcak_Graphene&diff=3984&oldid=prev 2021-05-25T18:14:59Z

<p>Created page with &quot;== Citation == Palovcak, E.; Wang, F.; Zheng, S. Q.; Yu, Z.; Li, S.; Betegon, M.; Bulkley, D.; Agard, D. A. &amp; Cheng, Y. A simple and robust procedure for preparing graphe...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Palovcak, E.; Wang, F.; Zheng, S. Q.; Yu, Z.; Li, S.; Betegon, M.; Bulkley, D.; Agard, D. A. &amp;amp; Cheng, Y. A simple and robust procedure for preparing graphene-oxide cryo-EM grids. J. Structural Biology, 2018, 204, 80-84 <br /> <br /> == Abstract ==<br /> <br /> Graphene oxide (GO) sheets have been used successfully as a supporting substrate film in several recent cryogenic&lt;br&gt;electron-microscopy (cryo-EM) studies of challenging biological macromolecules. However, difficulties in&lt;br&gt;preparing GO-covered holey carbon EM grids have limited their widespread use. Here, we report a simple and&lt;br&gt;robust method for covering holey carbon EM grids with GO sheets and demonstrate that these grids can be used&lt;br&gt;for high-resolution single particle cryo-EM. GO substrates adhere macromolecules, allowing cryo-EM grid preparation&lt;br&gt;with lower specimen concentrations and provide partial protection from the air-water interface.&lt;br&gt;Additionally, the signal of the GO lattice beneath the frozen-hydrated specimen can be discerned in many&lt;br&gt;motion-corrected micrographs, providing a high-resolution fiducial for evaluating beam-induced motion correction. <br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.sciencedirect.com/science/article/pii/S1047847718301655<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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