https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2018Sieben_Multicolor 2026-05-24T22:00:41Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2018Sieben_Multicolor&diff=3441&oldid=prev 2019-01-31T07:43:50Z

<p>Created page with &quot;== Citation == Sieben, C.; Banterle, N.; Douglass, K. M.; Gönczy, P. &amp; Manley, S. Multicolor single-particle reconstruction of protein complexes. Nature methods, 2018, 1...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Sieben, C.; Banterle, N.; Douglass, K. M.; Gönczy, P. &amp;amp; Manley, S. Multicolor single-particle reconstruction of protein complexes. Nature methods, 2018, 15, 777-780 <br /> <br /> == Abstract ==<br /> <br /> Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and coaligns multiple proteins from 2D super-resolution fluorescence images. To demonstrate our method, we generated multicolor 3D reconstructions of several proteins within the human centriole, which revealed their relative locations, dimensions and orientations. <br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.nature.com/articles/s41592-018-0140-x.pdf<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

WikiSysop