https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2018Sorzano_Highres 2026-06-13T12:17:43Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2018Sorzano_Highres&diff=3406&oldid=prev 2019-01-02T06:29:39Z

<p>Created page with &quot;== Citation == Sorzano, C. O. S.; Vargas, J.; de la Rosa-Trev?n, J. M.; Jim?nez-Moreno, A.; Melero, R.; Mart?nez, M.; Conesa, P.; Vilas, J. L.; Marabini, R. and Carazo, J. M....&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Sorzano, C. O. S.; Vargas, J.; de la Rosa-Trev?n, J. M.; Jim?nez-Moreno, A.; Melero, R.; Mart?nez, M.; Conesa, P.; Vilas, J. L.; Marabini, R. and Carazo, J. M. High-resolution reconstruction of Single Particles by Electron Microscopy. J. Structural Biology, 2018, 204, 329-337<br /> <br /> <br /> == Abstract ==<br /> <br /> The Map Challenge organized by the Electron Microscopy Data Bank has prompted the development of an Xmipp high resolution reconstruction protocol (which we will refer to as highres) that is integrated in the software platform Scipion. In this work we describe the details of the image angular alignment and map reconstruction steps in our new method. This algorithm is similar to the standard projection matching approach with some important modifications, especially in the area of detecting significant features in the reconstructed volume. We show that the new method is able to produce higher resolution maps than the current de facto standard as measured by the Fourier Shell Correlation, the Monogenic Local Resolution and EMRinger.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.sciencedirect.com/science/article/pii/S1047847718301989<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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