https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2018Wei_Grids 2026-06-13T12:17:18Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2018Wei_Grids&diff=3246&oldid=prev 2018-03-20T18:46:39Z

<p>Created page with &quot;== Citation == Wei, H.; Dandey, V. P.; Zhang, Z.; Raczkowski, A.; Rice, W. J.; Carragher, B. &amp; Potter, C. S. Optimizing &quot;self-wicking&quot; nanowire grids. Journal of structural b...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Wei, H.; Dandey, V. P.; Zhang, Z.; Raczkowski, A.; Rice, W. J.; Carragher, B. &amp; Potter, C. S. Optimizing &quot;self-wicking&quot; nanowire grids. Journal of structural biology, 2018<br /> <br /> == Abstract ==<br /> <br /> We have developed a self-blotting TEM grid for use with a novel instrument for vitrifying samples for cryo-electron microscopy (cryoEM). Nanowires are grown on the copper surface of the grid using a simple chemical reaction and the opposite smooth side is used to adhere to a holey sample substrate support, for example carbon or gold. When small volumes of sample are applied to the nanowire grids the wires effectively act as blotting paper to rapidly wick away the liquid, leaving behind a thin film. In this technical note, we present a detailed description of how we make these grids using a variety of substrates fenestrated with either lacey or regularly spaced holes. We explain how we characterize the quality of the grids and we describe their behavior under a variety of conditions.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.sciencedirect.com/science/article/pii/S1047847718300017<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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