WikiSysop: Created page with "== Citation == Beckers, M.; Jakobi, A. J.; Sachse, C. Thresholding of cryo-EM density maps by false discovery rate control. IUCrJ, 2019, 6, 18-33 == Abstract == Cryo-EM no..."

2020-07-24T07:07:30Z

Created page with "== Citation == Beckers, M.; Jakobi, A. J.; Sachse, C. Thresholding of cryo-EM density maps by false discovery rate control. IUCrJ, 2019, 6, 18-33 == Abstract == Cryo-EM no..."

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== Citation ==

Beckers, M.; Jakobi, A. J.; Sachse, C. Thresholding of cryo-EM density maps by false discovery rate control. IUCrJ, 2019, 6, 18-33

== Abstract ==

Cryo-EM now commonly generates close-to-atomic resolution as well as intermediate resolution maps from macromolecules observed in isolation and in situ. Interpreting these maps remains a challenging task owing to poor signal in the highest resolution shells and the necessity to select a threshold for density analysis. In order to facilitate this process, a statistical framework for the generation of confidence maps by multiple hypothesis testing and false discovery rate (FDR) control has been developed. In this way, three-dimensional confidence maps contain signal separated from background noise in the form of local detection rates of EM density values. It is demonstrated that confidence maps and FDR-based thresholding can be used for the interpretation of near-atomic resolution single-particle structures as well as lower resolution maps determined by subtomogram averaging. Confidence maps represent a conservative way of interpreting molecular structures owing to minimized noise. At the same time they provide a detection error with respect to background noise, which is associated with the density and is particularly beneficial for the interpretation of weaker cryo-EM densities in cases of conformational flexibility and lower occupancy of bound molecules and ions in the structure.

== Keywords ==

== Links ==

https://journals.iucr.org/m/issues/2019/01/00/pw5002/index.html

== Related software ==

== Related methods ==

== Comments ==

2019Beckers FDR - Revision history

WikiSysop: Created page with "== Citation == Beckers, M.; Jakobi, A. J.; Sachse, C. Thresholding of cryo-EM density maps by false discovery rate control. IUCrJ, 2019, 6, 18-33 == Abstract == Cryo-EM no..."