https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2019Bonomi_Multiscale 2026-05-24T20:15:38Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2019Bonomi_Multiscale&diff=3471&oldid=prev 2019-04-16T05:32:54Z

<p>Created page with &quot;== Citation == Bonomi, M.; Hanot, S.; Greenberg, C. H.; Sali, A.; Nilges, M.; Vendruscolo, M. &amp; Pellarin, R. Bayesian Weighing of Electron Cryo-Microscopy Data for Integrativ...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Bonomi, M.; Hanot, S.; Greenberg, C. H.; Sali, A.; Nilges, M.; Vendruscolo, M. &amp; Pellarin, R. Bayesian Weighing of Electron Cryo-Microscopy Data for Integrative Structural Modeling. Structure, 2019, 27, 175-188.e6 <br /> <br /> == Abstract ==<br /> <br /> Cryo-electron microscopy (cryo-EM) has become a mainstream technique for determining the structures of complex biological systems. However, accurate integrative structural modeling has been hampered by the challenges in objectively weighing cryo-EM data against other sources of information due to the presence of random and systematic errors, as well as correlations, in the data. To address these challenges, we introduce a Bayesian scoring function that efficiently and accurately ranks alternative structural models of a macromolecular system based on their consistency with a cryo-EM density map as well as other experimental and prior information. The accuracy of this approach is benchmarked using complexes of known structure and illustrated in three applications: the structural determination of the GroEL/GroES, RNA polymerase II, and exosome complexes. The approach is implemented in the open-source Integrative Modeling Platform (http://integrativemodeling.org), thus enabling integrative structure determination by combining cryo-EM data with other sources of information.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.sciencedirect.com/science/article/pii/S096921261830337X<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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