WikiSysop: Created page with "== Citation == Herzik, M. A.; Fraser, J. S. & Lander, G. C. A Multi-model Approach to Assessing Local and Global Cryo-EM Map Quality. Structure, 2019, 27, 344-358.e3 == Abs..."

2019-04-17T17:30:43Z

Created page with "== Citation == Herzik, M. A.; Fraser, J. S. & Lander, G. C. A Multi-model Approach to Assessing Local and Global Cryo-EM Map Quality. Structure, 2019, 27, 344-358.e3 == Abs..."

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== Citation ==

Herzik, M. A.; Fraser, J. S. & Lander, G. C. A Multi-model Approach to Assessing Local and Global Cryo-EM Map Quality. Structure, 2019, 27, 344-358.e3

== Abstract ==

There does not currently exist a standardized indicator of how well cryo-EM-derived models represent the density from which they were generated. We present a straightforward methodology that utilizes freely available tools to generate a suite of independent models and to evaluate their convergence in an EM density. These analyses provide both a quantitative and qualitative assessment of the precision of the models and their representation of the density, respectively, while concurrently providing a platform for assessing both global and local EM map quality. We further use standardized datasets to provide an expected deviation within a suite of models refined against EM maps reported to be at 5 Å resolution or better. Associating multiple atomic models with a deposited EM map provides a rapid and accessible reporter of convergence, a strong indicator of highly resolved molecular detail, and is an important step toward an FSC-independent assessment of map and model quality.

== Keywords ==

== Links ==

https://www.sciencedirect.com/science/article/pii/S0969212618303642

== Related software ==

== Related methods ==

== Comments ==

2019Herzik Multimodel - Revision history

WikiSysop: Created page with "== Citation == Herzik, M. A.; Fraser, J. S. & Lander, G. C. A Multi-model Approach to Assessing Local and Global Cryo-EM Map Quality. Structure, 2019, 27, 344-358.e3 == Abs..."