WikiSysop: Created page with "== Citation == Schorb, M.; Haberbosch, I.; Hagen, W. J. H.; Schwab, Y. & Mastronarde, D. N. Software tools for automated transmission electron microscopy. Nature methods,..."

2020-07-20T09:46:34Z

Created page with "== Citation == Schorb, M.; Haberbosch, I.; Hagen, W. J. H.; Schwab, Y. & Mastronarde, D. N. Software tools for automated transmission electron microscopy. Nature methods,..."

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== Citation ==

Schorb, M.; Haberbosch, I.; Hagen, W. J. H.; Schwab, Y. & Mastronarde, D. N. Software tools for automated transmission electron microscopy. Nature methods, 2019, 16, 471-477

== Abstract ==

The demand for high-throughput data collection in electron microscopy is increasing for applications in structural and cellular biology. Here we present a combination of software tools that enable automated acquisition guided by image analysis for a variety of transmission electron microscopy acquisition schemes. SerialEM controls microscopes and detectors and can trigger automated tasks at multiple positions with high flexibility. Py-EM interfaces with SerialEM to enact specimen-specific image-analysis pipelines that enable feedback microscopy. As example applications, we demonstrate dose reduction in cryo-electron microscopy experiments, fully automated acquisition of every cell in a plastic section and automated targeting on serial sections for 3D volume imaging across multiple grids.

== Keywords ==

== Links ==

https://www.nature.com/articles/s41592-019-0396-9

== Related software ==

== Related methods ==

== Comments ==

2019Schorb ET - Revision history

WikiSysop: Created page with "== Citation == Schorb, M.; Haberbosch, I.; Hagen, W. J. H.; Schwab, Y. & Mastronarde, D. N. Software tools for automated transmission electron microscopy. Nature methods,..."