WikiSysop: Created page with "== Citation == Thompson, R. F.; Iadanza, M. G.; Hesketh, E. L.; Rawson, S. and Ranson, N. A. Collection, pre-processing and on-the-fly analysis of data for high-resolution, s..."

2019-01-17T08:11:53Z

Created page with "== Citation == Thompson, R. F.; Iadanza, M. G.; Hesketh, E. L.; Rawson, S. and Ranson, N. A. Collection, pre-processing and on-the-fly analysis of data for high-resolution, s..."

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== Citation ==

Thompson, R. F.; Iadanza, M. G.; Hesketh, E. L.; Rawson, S. and Ranson, N. A. Collection, pre-processing and on-the-fly analysis of data for high-resolution, single-particle cryo-electron microscopy. Nature protocols, 2019, 14, 100-118

== Abstract ==

The dramatic growth in the use of cryo-electron microscopy (cryo-EM) to generate high-resolution structures of macromolecular complexes has changed the landscape of structural biology. The majority of structures deposited in the Electron Microscopy Data Bank (EMDB) at higher than 4-Å resolution were collected on Titan Krios microscopes. Although the pipeline for single-particle data collection is becoming routine, there is much variation in how sessions are set up. Furthermore, when collection is under way, there are a range of approaches for efficiently moving and pre-processing these data. Here, we present a standard operating procedure for single-particle data collection with Thermo Fisher Scientific EPU software, using the two most common direct electron detectors (the Thermo Fisher Scientific Falcon 3 (F3EC) and the Gatan K2), as well as a strategy for structuring these data to enable efficient pre-processing and on-the-fly monitoring of data collection. This protocol takes 3-6 h to set up a typical automated data collection session.

== Keywords ==

== Links ==

https://www.nature.com/articles/s41596-018-0084-8.pdf

== Related software ==

== Related methods ==

== Comments ==

2019Thompson Protocol - Revision history

WikiSysop: Created page with "== Citation == Thompson, R. F.; Iadanza, M. G.; Hesketh, E. L.; Rawson, S. and Ranson, N. A. Collection, pre-processing and on-the-fly analysis of data for high-resolution, s..."