WikiSysop: Created page with "== Citation == Wilkinson, M.; Kumar, A. & Casañal, A. Methods for merging data sets in electron cryo-microscopy Acta Crystallographica Section D: Structural Biology, Intern..."

2019-08-28T05:48:12Z

Created page with "== Citation == Wilkinson, M.; Kumar, A. & Casañal, A. Methods for merging data sets in electron cryo-microscopy Acta Crystallographica Section D: Structural Biology, Intern..."

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== Citation ==

Wilkinson, M.; Kumar, A. & Casañal, A.
Methods for merging data sets in electron cryo-microscopy
Acta Crystallographica Section D: Structural Biology, International Union of Crystallography, 2019 , 75

== Abstract ==

Recent developments have resulted in electron cryo-microscopy (cryo-EM)
becoming a useful tool for the structure determination of biological
macromolecules. For samples containing inherent flexibility, heterogeneity or
preferred orientation, the collection of extensive cryo-EM data using several
conditions and microscopes is often required. In such a scenario, merging cryo-
EM data sets is advantageous because it allows improved three-dimensional
reconstructions to be obtained. Since data sets are not always collected with the
same pixel size, merging data can be challenging. Here, two methods to combine
cryo-EM data are described. Both involve the calculation of a rescaling factor
from independent data sets. The effects of errors in the scaling factor on the
results of data merging are also estimated. The methods described here provide
a guideline for cryo-EM users who wish to combine data sets from the same type
of microscope and detector.

== Keywords ==

== Links ==

http://scripts.iucr.org/cgi-bin/paper?ic5109

== Related software ==

== Related methods ==

== Comments ==

2019Wilkinson Scales - Revision history

WikiSysop: Created page with "== Citation == Wilkinson, M.; Kumar, A. & Casañal, A. Methods for merging data sets in electron cryo-microscopy Acta Crystallographica Section D: Structural Biology, Intern..."