https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2020Cianfrocco_Wrong 2026-06-13T12:16:54Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2020Cianfrocco_Wrong&diff=3626&oldid=prev 2020-03-11T18:58:07Z

<p>Created page with &quot;== Citation == Cianfrocco, M. A. &amp; Kellogg, E. H. What Could Go Wrong? A Practical Guide to Single-Particle Cryo-EM: From Biochemistry to Atomic Models. Journal of chemical...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Cianfrocco, M. A. &amp; Kellogg, E. H.<br /> What Could Go Wrong? A Practical Guide to Single-Particle Cryo-EM: From Biochemistry to Atomic Models. <br /> Journal of chemical information and modeling, 2020 <br /> <br /> == Abstract ==<br /> <br /> Cryo-electron microscopy (cryo-EM) has enjoyed explosive recent growth due to revolutionary advances in hardware and software, resulting in a steady stream of long-awaited, high-resolution structures with unprecedented atomic detail. With this comes an increased number of microscopes, cryo-EM facilities, and scientists eager to leverage the ability to determine protein structures without crystallization. However, numerous pitfalls and considerations beset the path toward high-resolution structures and are not necessarily obvious from literature surveys. Here, we detail the most common misconceptions when initiating a cryo-EM project and common technical hurdles, as well as their solutions, and we conclude with a vision for the future of this exciting field.<br /> <br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://pubs.acs.org/doi/10.1021/acs.jcim.9b01178<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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