WikiSysop: Created page with "== Citation == Guo, H.; Franken, E.; Deng, Y.; Benlekbir, S.; Singla Lezcano, G.; Janssen, B.; Yu, L.; Ripstein, Z.; Tan, Y.; Rubinstein, J. Electron-event representation dat..."

2020-08-13T07:25:32Z

Created page with "== Citation == Guo, H.; Franken, E.; Deng, Y.; Benlekbir, S.; Singla Lezcano, G.; Janssen, B.; Yu, L.; Ripstein, Z.; Tan, Y.; Rubinstein, J. Electron-event representation dat..."

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== Citation ==

Guo, H.; Franken, E.; Deng, Y.; Benlekbir, S.; Singla Lezcano, G.; Janssen, B.; Yu, L.; Ripstein, Z.; Tan, Y.; Rubinstein, J. Electron-event representation data enable efficient cryoEM file storage with full preservation of spatial and temporal resolution. IUCrJ, International Union of Crystallography, 2020, 7

== Abstract ==

Direct detector device (DDD) cameras have revolutionized electron cryomicroscopy
(cryoEM) with their high detective quantum efficiency (DQE) and output
of movie data. A high ratio of camera frame rate (frames per second) to camera
exposure rate (electrons per pixel per second) allows electron counting, which
further improves the DQE and enables the recording of super-resolution
information. Movie output also allows the correction of specimen movement
and compensation for radiation damage. However, these movies come at the
cost of producing large volumes of data. It is common practice to sum groups of
successive camera frames to reduce the final frame rate, and therefore the file
size, to one suitable for storage and image processing. This reduction in the
temporal resolution of the camera requires decisions to be made during data
acquisition that may result in the loss of information that could have been
advantageous during image analysis. Here, experimental analysis of a new
electron-event representation (EER) data format for electron-counting DDD
movies is presented, which is enabled by new hardware developed by Thermo
Fisher Scientific for their Falcon DDD cameras. This format enables the
recording of DDD movies at the raw camera frame rate without sacrificing
either spatial or temporal resolution. Experimental data demonstrate that
the method retains super-resolution information and allows the correction of
specimen movement at the physical frame rate of the camera while maintaining
manageable file sizes. The EER format will enable the development of new
methods that can utilize the full spatial and temporal resolution of DDD
cameras.

== Keywords ==

== Links ==

https://journals.iucr.org/m/issues/2020/05/00/fq5014/index.html

== Related software ==

== Related methods ==

== Comments ==

2020Guo EER - Revision history

WikiSysop: Created page with "== Citation == Guo, H.; Franken, E.; Deng, Y.; Benlekbir, S.; Singla Lezcano, G.; Janssen, B.; Yu, L.; Ripstein, Z.; Tan, Y.; Rubinstein, J. Electron-event representation dat..."