WikiSysop: Created page with "== Citation == Hoh, S. W.; Burnley, T.; Cowtan, K. Current approaches for automated model building into cryo-EM maps using Buccaneer with CCP-EM. Acta crystallographica. Sect..."

2020-07-24T07:18:10Z

Created page with "== Citation == Hoh, S. W.; Burnley, T.; Cowtan, K. Current approaches for automated model building into cryo-EM maps using Buccaneer with CCP-EM. Acta crystallographica. Sect..."

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== Citation ==

Hoh, S. W.; Burnley, T.; Cowtan, K. Current approaches for automated model building into cryo-EM maps using Buccaneer with CCP-EM. Acta crystallographica. Section D, Structural biology, 2020, 76, 531-541

== Abstract ==

This work focuses on the use of the existing protein-model-building software Buccaneer to provide structural interpretation of electron cryo-microscopy (cryo-EM) maps. Originally developed for application to X-ray crystallography, the necessary steps to optimise the usage of Buccaneer with cryo-EM maps are shown. This approach has been applied to the data sets of 208 cryo-EM maps with resolutions of better than 4 Å. The results obtained also show an evident improvement in the sequencing step when the initial reference map and model used for crystallographic cases are replaced by a cryo-EM reference. All other necessary changes to settings in Buccaneer are implemented in the model-building pipeline from within the CCP-EM interface (as of version 1.4.0).

== Keywords ==

== Links ==

https://scripts.iucr.org/cgi-bin/paper?id5008

== Related software ==

== Related methods ==

== Comments ==

2020Hoh Buccaneer - Revision history

WikiSysop: Created page with "== Citation == Hoh, S. W.; Burnley, T.; Cowtan, K. Current approaches for automated model building into cryo-EM maps using Buccaneer with CCP-EM. Acta crystallographica. Sect..."