https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2020Song_Tygress2020Song Tygress - Revision history2026-05-24T20:20:57ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=2020Song_Tygress&diff=3624&oldid=prevWikiSysop: Created page with "== Citation == Song, K.; Shang, Z.; Fu, X.; Lou, X.; Grigorieff, N. & Nicastro, D. In situ structure determination at nanometer resolution using TYGRESS. Nature methods, 202..."2020-03-03T21:26:59Z<p>Created page with "== Citation == Song, K.; Shang, Z.; Fu, X.; Lou, X.; Grigorieff, N. & Nicastro, D. In situ structure determination at nanometer resolution using TYGRESS. Nature methods, 202..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
<br />
Song, K.; Shang, Z.; Fu, X.; Lou, X.; Grigorieff, N. & Nicastro, D.<br />
In situ structure determination at nanometer resolution using TYGRESS. <br />
Nature methods, 2020 , 17 , 201-208 <br />
<br />
== Abstract ==<br />
<br />
The resolution of subtomogram averages calculated from cryo-electron tomograms (cryo-ET) of crowded cellular environments is often limited owing to signal loss in, and misalignment of, the subtomograms. By contrast, single-particle cryo-electron microscopy (SP-cryo-EM) routinely reaches near-atomic resolution of isolated complexes. We report a method called 'tomography-guided 3D reconstruction of subcellular structures' (TYGRESS) that is a hybrid of cryo-ET and SP-cryo-EM, and is able to achieve close-to-nanometer resolution of complexes inside crowded cellular environments. TYGRESS combines the advantages of SP-cryo-EM (images with good signal-to-noise ratio and contrast, as well as minimal radiation damage) and subtomogram averaging (three-dimensional alignment of macromolecules in a complex sample). Using TYGRESS, we determined the structure of the intact ciliary axoneme with up to resolution of 12 Å. These results reveal many structural details that were not visible by cryo-ET alone. TYGRESS is generally applicable to cellular complexes that are amenable to subtomogram averaging.<br />
<br />
== Keywords ==<br />
<br />
== Links ==<br />
<br />
https://www.nature.com/articles/s41592-019-0651-0<br />
<br />
== Related software ==<br />
<br />
== Related methods ==<br />
<br />
== Comments ==</div>WikiSysop