https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2020Stabrin_Transphire 2026-05-24T22:01:04Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2020Stabrin_Transphire&diff=4006&oldid=prev 2021-05-27T08:54:14Z

<p>Created page with &quot;== Citation == Stabrin, M.; Schoenfeld, F.; Wagner, T.; Pospich, S.; Gatsogiannis, C. &amp; Raunser, S. TranSPHIRE: automated and feedback-optimized on-the-fly processing for...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Stabrin, M.; Schoenfeld, F.; Wagner, T.; Pospich, S.; Gatsogiannis, C. &amp;amp; Raunser, S. TranSPHIRE: automated and feedback-optimized on-the-fly processing for cryo-EM. Nature communications, 2020, 11, 5716 <br /> <br /> == Abstract ==<br /> <br /> Single particle cryo-EM requires full automation to allow high-throughput structure determination. Although software packages exist where parts of the cryo-EM pipeline are automated, a complete solution that offers reliable on-the-fly processing, resulting in high-resolution structures, does not exist. Here we present TranSPHIRE: A software package for fully-automated processing of cryo-EM datasets during data acquisition. TranSPHIRE transfers data from the microscope, automatically applies the common pre-processing steps, picks particles, performs 2D clustering, and 3D refinement parallel to image recording. Importantly, TranSPHIRE introduces a machine learning-based feedback loop to re-train its picking model to adapt to any given data set live during processing. This elegant approach enables TranSPHIRE to process data more effectively, producing high-quality particle stacks. TranSPHIRE collects and displays all metrics and microscope settings to allow users to quickly evaluate data during acquisition. TranSPHIRE can run on a single work station and also includes the automated processing of filaments. <br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.nature.com/articles/s41467-020-19513-2<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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