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Created page with "== Citation == Venkatakrishnan, S.; Juneja, P. & O’Neill, H. Model-based Reconstruction for Single Particle Cryo-Electron Microscopy. 2020 54th Asilomar Conference on S..."

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== Citation ==

Venkatakrishnan, S.; Juneja, P. & O’Neill, H. Model-based Reconstruction for Single Particle Cryo-Electron Microscopy. 2020 54th Asilomar Conference on Signals, Systems, and Computers, 2020, 1390-1394

== Abstract ==

Single particle cryo-electron microscopy is a vital
tool for 3D characterization of protein structures. A typical
workflow involves acquiring projection images of a collection of
randomly oriented particles, picking and classifying individual
particle projections by orientation, and finally using the individual particle projections to reconstruct a 3D map of the electron
density profile. The reconstruction is challenging because of the
low signal-to-noise ratio of the data, the unknown orientation of
the particles, and the sparsity of data especially when dealing
with flexible proteins where there may not be sufficient data
corresponding to each class to obtain an accurate reconstruction
using standard algorithms. In this paper we present a model based image reconstruction technique that uses a regularized
cost function to reconstruct the 3D density map by assuming
known orientations for the particles. Our method casts the
reconstruction as minimizing a cost function involving a novel
forward model term that accounts for the contrast transfer
function of the microscope, the orientation of the particles and the
center of rotation offsets. We combine the forward model term
with a regularizer that enforces desirable properties in the volume
to be reconstructed. Using simulated data, we demonstrate how
our method can significantly improve upon the typically used
approach.

== Keywords ==

== Links ==

https://ieeexplore.ieee.org/stamp/stamp.jsp?arnumber=9443387

== Related software ==

== Related methods ==

== Comments ==

2020Venkatakrishnan MBIR - Revision history

WikiSysop: Created page with "== Citation == Venkatakrishnan, S.; Juneja, P. & O’Neill, H. Model-based Reconstruction for Single Particle Cryo-Electron Microscopy. 2020 54th Asilomar Conference on S..."