https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2020Wu_Review 2026-06-13T12:14:00Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2020Wu_Review&diff=3800&oldid=prev 2020-08-12T07:50:27Z

<p>Created page with &quot;== Citation == Wu, M.; Lander, G. C. How low can we go? Structure determination of small biological complexes using single-particle cryo-EM. Current Opinion in Structural Bio...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Wu, M.; Lander, G. C. How low can we go? Structure determination of small biological complexes using single-particle cryo-EM. Current Opinion in Structural Biology, 2020, 64, 9-16<br /> <br /> == Abstract ==<br /> For decades, high-resolution structural studies of biological<br /> macromolecules with masses of &lt;200 kDa by cryo-EM singleparticle<br /> analysis were considered infeasible. It was not until<br /> several years after the advent of direct detectors that the<br /> overlooked potential of cryo-EM for studying small complexes<br /> was first realized. Subsequent advances in sample preparation,<br /> imaging, and data processing algorithms have improved our<br /> ability to visualize small biological targets. In the past two years<br /> alone, nearly two hundred high-resolution structures have been<br /> determined of small (&lt;200 kDa) macromolecules, the smallest<br /> being approximately 39 kDa in molecular weight. Here we<br /> summarize some salient lessons and strategies for cryo-EM<br /> studies of small biological complexes, and also consider future<br /> prospects for routine structure determination. <br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.sciencedirect.com/science/article/pii/S0959440X20300841<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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