https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2020Yoder_TimeResolved 2026-06-13T12:10:07Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2020Yoder_TimeResolved&diff=3885&oldid=prev 2021-02-12T07:43:15Z

<p>Created page with &quot;== Citation == Yoder, N.; Jalali-Yazdi, F.; Noreng, S.; Houser, A.; Baconguis, I.; Gouaux, E. Light-coupled cryo-plunger for time-resolved cryo-EM. Journal of structural biol...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Yoder, N.; Jalali-Yazdi, F.; Noreng, S.; Houser, A.; Baconguis, I.; Gouaux, E. Light-coupled cryo-plunger for time-resolved cryo-EM. Journal of structural biology, 2020, 212, 107624 <br /> <br /> == Abstract ==<br /> <br /> Proteins are dynamic molecules that can undergo rapid conformational rearrangements in response to stimuli. These structural changes are often critical to protein function, and thus elucidating time-dependent conformational landscapes has been a long-standing goal of structural biology. To harness the power of single particle cryo-EM methods to enable &#039;time-resolved&#039; structure determination, we have developed a light-coupled cryo-plunger that pairs flash-photolysis of caged ligands with rapid sample vitrification. The &#039;flash-plunger&#039; consists of a high-power ultraviolet LED coupled with focusing optics and a motorized linear actuator, enabling the user to immobilize protein targets in vitreous ice within a programmable time window - as short as tens of milliseconds - after stimulus delivery. The flash-plunger is a simple, inexpensive and flexible tool to explore short-lived conformational states previously unobtainable by conventional sample preparation methods. <br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.sciencedirect.com/science/article/pii/S1047847720301970<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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