https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2021Budell_TimeResolved 2026-05-24T18:05:12Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2021Budell_TimeResolved&diff=3944&oldid=prev 2021-04-12T14:32:24Z

<p>Created page with &quot;== Citation == Budell, W. C.; Allegri, L.; Dandey, V.; Potter, C. S. &amp; Carragher, B. Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Ro...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Budell, W. C.; Allegri, L.; Dandey, V.; Potter, C. S. &amp;amp; Carragher, B. Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton. Journal of visualized experiments: JoVE, 2021<br /> <br /> == Abstract ==<br /> <br /> The capture of short-lived molecular states triggered by the early encounter of two or more interacting particles continues to be an experimental challenge of great interest to the field of cryo-electron microscopy (cryo-EM). A few methodological strategies have been developed that support these &quot;time-resolved&quot; studies, one of which, Spotiton-a novel robotic system-combines the dispensing of picoliter-sized sample droplets with precise temporal and spatial control. The time-resolved Spotiton workflow offers a uniquely efficient approach to interrogate early structural rearrangements from minimal sample volume. Fired from independently controlled piezoelectric dispensers, two samples land and rapidly mix on a nanowire EM grid as it plunges toward the cryogen. Potentially hundreds of grids can be prepared in rapid succession from only a few microliters of a sample. Here, a detailed step-by-step protocol of the operation of the Spotiton system is presented with a focus on troubleshooting specific problems that arise during grid preparation. <br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.jove.com/t/62271/cryo-electron-microscopic-grid-preparation-for-time-resolved-studies<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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