https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2021Burt_RWD 2026-06-13T12:13:27Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2021Burt_RWD&diff=4077&oldid=prev 2021-09-20T08:27:52Z

<p>Created page with &quot;== Citation == Burt, A.; Gaifas, L.; Dendooven, T. &amp; Gutsche, I. A flexible framework for multi-particle refinement in cryo-electron tomography. PLoS biology, 2021, 19, e...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Burt, A.; Gaifas, L.; Dendooven, T. &amp;amp; Gutsche, I. A flexible framework for multi-particle refinement in cryo-electron tomography. PLoS biology, 2021, 19, e3001319 <br /> <br /> == Abstract ==<br /> <br /> Cryo-electron tomography (cryo-ET) and subtomogram averaging (STA) are increasingly used for macromolecular structure determination in situ. Here, we introduce a set of computational tools and resources designed to enable flexible approaches to STA through increased automation and simplified metadata handling. We create a bidirectional interface between the Dynamo software package and the Warp-Relion-M pipeline, providing a framework for ab initio and geometrical approaches to multiparticle refinement in M. We illustrate the power of working within this framework by applying it to EMPIAR-10164, a publicly available dataset containing immature HIV-1 virus-like particles (VLPs), and a challenging in situ dataset containing chemosensory arrays in bacterial minicells. Additionally, we provide a comprehensive, step-by-step guide to obtaining a 3.4-Å reconstruction from EMPIAR-10164. The guide is hosted on https://teamtomo.org/, a collaborative online platform we establish for sharing knowledge about cryo-ET. <br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3001319<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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