https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2021Cheng_Native2021Cheng Native - Revision history2026-05-24T19:35:26ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=2021Cheng_Native&diff=4507&oldid=prevWikiSysop: Created page with "== Citation == Cheng, Jing / Li, Bufan / Si, Long / Zhang, Xinzheng. Determining structures in a native environment using single-particle cryoelectron microscopy images. 2021..."2024-01-08T07:11:59Z<p>Created page with "== Citation == Cheng, Jing / Li, Bufan / Si, Long / Zhang, Xinzheng. Determining structures in a native environment using single-particle cryoelectron microscopy images. 2021..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
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Cheng, Jing / Li, Bufan / Si, Long / Zhang, Xinzheng. Determining structures in a native environment using single-particle cryoelectron microscopy images. 2021. The Innovation, Vol. 2, No. 4, p. 100166<br />
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== Abstract ==<br />
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Cryo-electron tomography is a powerful tool for structure determination<br />
in the native environment. However, this method requires the acquisition<br />
of tilt series, which is time-consuming and severely slows structure<br />
determination. By treating the densities of non-target protein as non-<br />
Gaussian noise, we developed a new target function that greatly improves<br />
the efficiency of recognizing the target protein in a single cryoelectron<br />
microscopy image. Moreover, we developed a sorting function<br />
that effectively eliminates the model dependence and improved the resolution<br />
during the subsequent structure refinement procedure. By<br />
eliminating model bias, our method allows using homolog proteins as<br />
models to recognize the target proteins in a complex context. Together,<br />
we developed an in situ single-particle analysis method. Our method was<br />
successfully applied to solve structures of glycoproteins on the surface<br />
of a non-icosahedral virus and Rubisco inside the carboxysome. Both<br />
data were collected within 24 h, thus allowing fast and simple structural<br />
determination.<br />
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== Keywords ==<br />
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== Links ==<br />
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https://www.sciencedirect.com/science/article/pii/S2666675821000916<br />
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== Related software ==<br />
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== Related methods ==<br />
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== Comments ==</div>WikiSysop