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2021-04-26T13:14:22Z

Created page with "== Citation == Chojnowski, G.; Sobolev, E.; Heuser, P. & Lamzin, V. S. The accuracy of protein models automatically built into cryo-EM maps with ARP/wARP. Acta crystallog..."

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== Citation ==

Chojnowski, G.; Sobolev, E.; Heuser, P. & Lamzin, V. S. The accuracy of protein models automatically built into cryo-EM maps with ARP/wARP. Acta crystallographica. Section D, Structural biology, 2021, 77, 142-150

== Abstract ==

Recent developments in cryogenic electron microscopy (cryo-EM) have enabled structural studies of large macromolecular complexes at resolutions previously only attainable using macromolecular crystallography. Although a number of methods can already assist in de novo building of models into high-resolution cryo-EM maps, automated and reliable map interpretation remains a challenge. Presented here is a systematic study of the accuracy of models built into cryo-EM maps using ARP/wARP. It is demonstrated that the local resolution is a good indicator of map interpretability, and for the majority of the test cases ARP/wARP correctly builds 90% of main-chain fragments in regions where the local resolution is 4.0 Å or better. It is also demonstrated that the coordinate accuracy for models built into cryo-EM maps is comparable to that of X-ray crystallographic models at similar local cryo-EM and crystallographic resolutions. The model accuracy also correlates with the refined atomic displacement parameters.

== Keywords ==

== Links ==

https://onlinelibrary.wiley.com/iucr/doi/10.1107/S2059798320016332

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== Related methods ==

== Comments ==

2021Chojnowski quality - Revision history

WikiSysop: Created page with "== Citation == Chojnowski, G.; Sobolev, E.; Heuser, P. & Lamzin, V. S. The accuracy of protein models automatically built into cryo-EM maps with ARP/wARP. Acta crystallog..."