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2021-04-29T13:37:49Z

Created page with "== Citation == Efremov, R. G. & Stroobants, A. Coma-corrected rapid single-particle cryo-EM data collection on the CRYO ARM 300. Acta Crystallographica Section D: Structu..."

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== Citation ==

Efremov, R. G. & Stroobants, A. Coma-corrected rapid single-particle cryo-EM data collection on the CRYO ARM 300. Acta Crystallographica Section D: Structural Biology, 2021, 77

== Abstract ==

Single-particle cryogenic electron microscopy has recently become a major method for determining the structures of proteins and protein complexes. This has markedly increased the demand for throughput of high-resolution electron microscopes, which are required to produce high-resolution images at high rates. An increase in data-collection throughput can be achieved by using large beamimage shifts combined with off-axis coma correction, enabling the acquisition of multiple images from a large area of the EM grid without moving the microscope stage. Here, the optical properties of the JEOL CRYO ARM 300 electron microscope equipped with a K3 camera were characterized under offaxis illumination conditions. It is shown that efficient coma correction can be achieved for beam-image shifts with an amplitude of at least 10 mm, enabling a routine throughput for data collection of between 6000 and 9000 images per day. Use of the benchmark for the rapid data-collection procedure (with beam-image shifts of up to 7 mm) on apoferritin resulted in a reconstruction at a resolution of 1.7 A°. This demonstrates that the rapid automated acquisition of high-resolution micrographs is possible using a CRYO ARM 300.

== Keywords ==

== Links ==

https://journals.iucr.org/d/issues/2021/05/00/id5009/

== Related software ==

== Related methods ==

== Comments ==

2021Efremov ComaCorrected - Revision history

WikiSysop: Created page with "== Citation == Efremov, R. G. & Stroobants, A. Coma-corrected rapid single-particle cryo-EM data collection on the CRYO ARM 300. Acta Crystallographica Section D: Structu..."