https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2021Jagota_GoldNanoparticles 2026-05-24T20:17:58Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2021Jagota_GoldNanoparticles&diff=4041&oldid=prev 2021-06-16T09:40:14Z

<p>Created page with &quot;== Citation == Jagota, M.; Townshend, R. J. L.; Kang, L.-W.; Bushnell, D. A.; Dror, R. O.; Kornberg, R. D. &amp; Azubel, M. Gold nanoparticles and tilt pairs to assess protei...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Jagota, M.; Townshend, R. J. L.; Kang, L.-W.; Bushnell, D. A.; Dror, R. O.; Kornberg, R. D. &amp;amp; Azubel, M. Gold nanoparticles and tilt pairs to assess protein flexibility by cryo-electron microscopy. Ultramicroscopy, 2021, 227, 113302 <br /> <br /> == Abstract ==<br /> <br /> A computational method was developed to recover the three-dimensional coordinates of gold nanoparticles specifically attached to a protein complex from tilt-pair images collected by electron microscopy. The program was tested on a simulated dataset and applied to a real dataset comprising tilt-pair images recorded by cryo electron microscopy of RNA polymerase II in a complex with four gold-labeled single-chain antibody fragments. The positions of the gold nanoparticles were determined, and comparison of the coordinates among the tetrameric particles revealed the range of motion within the protein complexes.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.sciencedirect.com/science/article/pii/S0304399121000899<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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