https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2021Jiang_MoAu 2026-05-24T20:17:58Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2021Jiang_MoAu&diff=4032&oldid=prev 2021-06-16T08:42:26Z

<p>Created page with &quot;== Citation == Jiang, X.; Xuan, S.; Zuckermann, R. N.; Glaeser, R. M.; Downing, K. H. &amp; Balsara, N. P. Minimizing Crinkling of Soft Specimens Using Holey Gold Films on Mo...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Jiang, X.; Xuan, S.; Zuckermann, R. N.; Glaeser, R. M.; Downing, K. H. &amp;amp; Balsara, N. P. Minimizing Crinkling of Soft Specimens Using Holey Gold Films on Molybdenum Grids for Cryogenic Electron Microscopy. Microscopy and microanalysis, 2021, 1-9 <br /> <br /> == Abstract ==<br /> <br /> We introduce a novel composite holey gold support that prevents cryo-crinkling and reduces beam-induced motion of soft specimens, building on the previously introduced all-gold support. The composite holey gold support for high-resolution cryogenic electron microscopy of soft crystalline membranes was fabricated in two steps. In the first step, a holey gold film was transferred on top of a molybdenum grid. In the second step, a continuous thin carbon film was transferred onto the holey gold film. This support (Au/Mo grid) was used to image crystalline synthetic polymer membranes. The low thermal expansion of Mo is not only expected to avoid cryo-crinkling of the membrane when the grids are cooled to cryogenic temperatures, but it may also act to reduce whatever crinkling existed even before cooling. The Au/Mo grid exhibits excellent performance with specimens tilted to 45°. This is demonstrated by quantifying beam-induced motion and differences in local defocus values. In addition, images of specimens on the Au/Mo grids that are tilted at 45° show high-resolution information of the crystalline membranes that, after lattice-unbending, extends beyond 1.5 Å in the direction perpendicular to the tilt axis. <br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/minimizing-crinkling-of-soft-specimens-using-holey-gold-films-on-molybdenum-grids-for-cryogenic-electron-microscopy/8351575CDD85B8E3E78C62879D089AAC<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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