WikiSysop: Created page with "== Citation == Méndez, J.; Garduño, E.; Carazo, J. M. & Sorzano, C. O. S. Identification of incorrectly oriented particles in Cryo-EM Single Particle Analys..."

2021-08-06T07:40:20Z

Created page with "== Citation == Méndez, J.; Garduño, E.; Carazo, J. M. & Sorzano, C. O. S. Identification of incorrectly oriented particles in Cryo-EM Single Particle Analys..."

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== Citation ==

Méndez, J.; Garduño, E.; Carazo, J. M. & Sorzano, C. O. S. Identification of incorrectly oriented particles in Cryo-EM Single Particle Analysis. J. Structural Biology, 2021, 213, 107771

== Abstract ==

The quality of a 3D map produced by the single-particle analysis method is highly dependent on an accurate assignment of orientations to the many experimental images. However, the problem’s complexity implies the presence of several local minima in the optimized goal functions. Consequently, validation methods to confirm the angular assignment are very useful to yield higher-resolution 3D maps. In this work, we present a graph-signal-processing-based methodology that analyzes the correlation landscape as a function of the orientation, an approach allowing the estimation of the assigned orientations’ reliability. Using this method, we may identify low-reliability images that probably incorrectly contribute to the final 3D reconstruction.

== Keywords ==

== Links ==

https://www.sciencedirect.com/science/article/pii/S1047847721000769

== Related software ==

== Related methods ==

== Comments ==

2021Mendez Graph - Revision history

WikiSysop: Created page with "== Citation == Méndez, J.; Garduño, E.; Carazo, J. M. & Sorzano, C. O. S. Identification of incorrectly oriented particles in Cryo-EM Single Particle Analys..."