https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2021Voss_Melting 2026-05-24T21:10:56Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2021Voss_Melting&diff=4043&oldid=prev 2021-07-14T06:28:33Z

<p>Created page with &quot;== Citation == Voss, J. M.; Harder, O. F.; Olshin, P. K.; Drabbels, M. &amp; Lorenz, U. J. Rapid Melting and Revitrification as an Approach to Microsecond Time-Resolved Cryo-...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Voss, J. M.; Harder, O. F.; Olshin, P. K.; Drabbels, M. &amp;amp; Lorenz, U. J. Rapid Melting and Revitrification as an Approach to Microsecond Time-Resolved Cryo-Electron Microscopy. Chemical Physics Letters, 2021, 778, 138812 <br /> <br /> == Abstract ==<br /> <br /> Proteins typically undergo dynamics on the microsecond to millisecond timescale, which is much faster than the time resolution of cryo-electron microscopy. Here, we propose a novel approach for microsecond time-resolved cryo-electron microscopy that involves melting a cryo specimen in situ with a laser beam. The sample remains liquid for the duration of the laser pulse, offering a tunable time window in which the dynamics of embedded particles can be induced in a liquid environment. After the laser pulse, the sample vitrifies, trapping particles in their transient configurations. As a proof of principle, we study the disassembly of particles after they incur structural damage.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.sciencedirect.com/science/article/pii/S0009261421004954<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

WikiSysop