WikiSysop: Created page with "== Citation == Wieferig, J.-P.; Mills, D. J. & Kühlbrandt, W. Devitrification reduces beam-induced movement in cryo-EM. IUCrJ, 2021, 8 == Abstract == As cryo-EM ap..."

2021-03-02T16:01:46Z

Created page with "== Citation == Wieferig, J.-P.; Mills, D. J. & Kühlbrandt, W. Devitrification reduces beam-induced movement in cryo-EM. IUCrJ, 2021, 8 == Abstract == As cryo-EM ap..."

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== Citation ==

Wieferig, J.-P.; Mills, D. J. & Kühlbrandt, W. Devitrification reduces beam-induced movement in cryo-EM. IUCrJ, 2021, 8

== Abstract ==

As cryo-EM approaches the physical resolution limits imposed by electron optics and radiation damage, it becomes increasingly urgent to address the issues that impede high-resolution structure determination of biological specimens. One of the persistent problems has been beam-induced movement, which occurs when the specimen is irradiated with high-energy electrons. Beam-induced movement results in image blurring and loss of high-resolution information. It is particularly severe for biological samples in unsupported thin films of vitreous water. By controlled devitrification of conventionally plunge-frozen samples, the suspended film of vitrified water was converted into cubic ice, a polycrystalline, mechanically stable solid. It is shown that compared with vitrified samples, devitrification reduces beam-induced movement in the first 5 e Å−2 of an exposure by a factor of ∼4, substantially enhancing the contribution of the initial, minimally damaged frames to a structure. A 3D apoferritin map reconstructed from the first frames of 20 000 particle images of devitrified samples resolved undamaged side chains. Devitrification of frozen-hydrated specimens helps to overcome beam-induced specimen motion in single-particle cryo-EM, as a further step towards realizing the full potential of cryo-EM for high-resolution structure determination.

== Keywords ==

== Links ==

https://journals.iucr.org/m/issues/2021/02/00/pw5016/index.html

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2021Wieferig Devitrification - Revision history

WikiSysop: Created page with "== Citation == Wieferig, J.-P.; Mills, D. J. & Kühlbrandt, W. Devitrification reduces beam-induced movement in cryo-EM. IUCrJ, 2021, 8 == Abstract == As cryo-EM ap..."