WikiSysop: Created page with "== Citation == Chojnowski, G.; Simpkin, A. J.; Leonardo, D. A.; Seifert-Davila, W.; Vivas-Ruiz, D. E.; Keegan, R. & Rigden, D. findMySequence: a neural-network-based appr..."

2021-12-30T18:42:56Z

Created page with "== Citation == Chojnowski, G.; Simpkin, A. J.; Leonardo, D. A.; Seifert-Davila, W.; Vivas-Ruiz, D. E.; Keegan, R. & Rigden, D. findMySequence: a neural-network-based appr..."

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== Citation ==

Chojnowski, G.; Simpkin, A. J.; Leonardo, D. A.; Seifert-Davila, W.; Vivas-Ruiz, D. E.; Keegan, R. & Rigden, D. findMySequence: a neural-network-based approach for identification of unknown proteins in X-ray crystallography and cryo-EM. IUCrJ, 2022, 9, 86-97

== Abstract ==

Although experimental protein-structure determination usually targets known proteins, chains of unknown sequence are often encountered. They can be purified from natural sources, appear as an unexpected fragment of a well characterized protein or appear as a contaminant. Regardless of the source of the problem, the unknown protein always requires characterization. Here, an automated pipeline is presented for the identification of protein sequences from cryo-EM reconstructions and crystallographic data. The method's application to characterize the crystal structure of an unknown protein purified from a snake venom is presented. It is also shown that the approach can be successfully applied to the identification of protein sequences and validation of sequence assignments in cryo-EM protein structures.

== Keywords ==

== Links ==

https://journals.iucr.org/m/issues/2022/01/00/pw5018/index.html

== Related software ==

== Related methods ==

== Comments ==

2022Chojnowski findMySeq - Revision history

WikiSysop: Created page with "== Citation == Chojnowski, G.; Simpkin, A. J.; Leonardo, D. A.; Seifert-Davila, W.; Vivas-Ruiz, D. E.; Keegan, R. & Rigden, D. findMySequence: a neural-network-based appr..."