https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2022Forster_Review 2026-05-24T22:00:35Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2022Forster_Review&diff=4191&oldid=prev 2022-03-23T21:21:53Z

<p>Created page with &quot;== Citation == Förster, Friedrich. Subtomogram analysis: The sum of a tomogram’s particles reveals molecular structure in situ. 2022, Journal of Structural Biology: X,...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> F&amp;ouml;rster, Friedrich. Subtomogram analysis: The sum of a tomogram’s particles reveals molecular structure in situ. 2022, Journal of Structural Biology: X, Vol. 6, p. 100063<br /> <br /> == Abstract ==<br /> <br /> Cryo-electron tomography is uniquely suited to provide insights into the molecular architecture of cells and tissue in the native state. While frozen hydrated specimens tolerate sufficient electron doses to distinguish different types of particles in a tomogram, the accumulating beam damage does not allow resolving their detailed molecular structure individually. Statistical methods for subtomogram averaging and classification that coherently enhance the signal of particles corresponding to copies of the same type of macromolecular allow obtaining much higher resolution insights into macromolecules. Here, I review the developments in subtomogram analysis at Wolfgang Baumeister’s laboratory that make the dream of structural biology in the native cell become reality. <br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.sciencedirect.com/science/article/pii/S2590152422000046<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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