https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2022He_EMBuild2022He EMBuild - Revision history2026-05-24T20:10:26ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=2022He_EMBuild&diff=4317&oldid=prevWikiSysop: Created page with "== Citation == He, Jiahua / Lin, Peicong / Chen, Ji / Cao, Hong / Huang, Sheng-You. Model building of protein complexes from intermediate-resolution cryo-EM maps with deep le..."2023-01-24T08:33:45Z<p>Created page with "== Citation == He, Jiahua / Lin, Peicong / Chen, Ji / Cao, Hong / Huang, Sheng-You. Model building of protein complexes from intermediate-resolution cryo-EM maps with deep le..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
<br />
He, Jiahua / Lin, Peicong / Chen, Ji / Cao, Hong / Huang, Sheng-You. Model building of protein complexes from intermediate-resolution cryo-EM maps with deep learning-guided automatic assembly, 2022. Nature Communications, Vol. 13, p. 4066 <br />
<br />
== Abstract ==<br />
<br />
Advances in microscopy instruments and image processing algorithms have led to an<br />
increasing number of cryo-electron microscopy (cryo-EM) maps. However, building accurate<br />
models into intermediate-resolution EM maps remains challenging and labor-intensive. Here,<br />
we propose an automatic model building method of multi-chain protein complexes from<br />
intermediate-resolution cryo-EM maps, named EMBuild, by integrating AlphaFold structure<br />
prediction, FFT-based global fitting, domain-based semi-flexible refinement, and graph-based<br />
iterative assembling on the main-chain probability map predicted by a deep convolutional<br />
network. EMBuild is extensively evaluated on diverse test sets of 47 single-particle EM maps<br />
at 4.0–8.0 Å resolution and 16 subtomogram averaging maps of cryo-ET data at 3.7–9.3 Å<br />
resolution, and compared with state-of-the-art approaches. We demonstrate that EMBuild is<br />
able to build high-quality complex structures that are comparably accurate to the manually<br />
built PDB structures from the cryo-EM maps. These results demonstrate the accuracy and<br />
reliability of EMBuild in automatic model building.<br />
<br />
== Keywords ==<br />
<br />
== Links ==<br />
<br />
https://www.nature.com/articles/s41467-022-31748-9<br />
<br />
== Related software ==<br />
<br />
== Related methods ==<br />
<br />
== Comments ==</div>WikiSysop