https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2022Scher_FIB 2026-05-24T22:01:28Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2022Scher_FIB&diff=4218&oldid=prev 2022-04-07T21:53:58Z

<p>Created page with &quot;== Citation == Scher, Nadav / Rechav, Katya / Paul-Gilloteaux, Perrine / Avinoam, Ori. Sample preparation and image registration for correlative cryo-FM and cryo-FIB-SEM of p...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Scher, Nadav / Rechav, Katya / Paul-Gilloteaux, Perrine / Avinoam, Ori. Sample preparation and image registration for correlative cryo-FM and cryo-FIB-SEM of plunge-frozen mammalian cells. 2022-03, STAR protocols, Vol. 3, p. 101142<br /> <br /> == Abstract ==<br /> <br /> We recently demonstrated how lipid droplets can serve as fiducials for correlating cryo-fluorescence microscopy (cryo-FM) and cryo-focused ion beam scanning electron microscopy (cryo-FIB-SEM) datasets of mammalian cells grown on grids. Here we describe a step-by-step protocol for correlative cryo-FM and cryo-FIB-SEM, starting from sample preparation of C2C12 cell line, followed by imaging with cryo-FM and cryo-FIB-SEM. Finally, we detail how to perform the 3D-correlation with sub-micron accuracy. For complete details on the use and execution of this profile, please refer to Scher et al. (2021). <br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://star-protocols.cell.com/protocols/1427<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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