WikiSysop: Created page with "== Citation == Basanta, Benjamin / Chen, Wenqian / Pride, Daniel E. / Lander, Gabriel C. Fabrication of Monolayer Graphene-Coated Grids forCryoelectron Microscopy. 2023. JoVE..."

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Created page with "== Citation == Basanta, Benjamin / Chen, Wenqian / Pride, Daniel E. / Lander, Gabriel C. Fabrication of Monolayer Graphene-Coated Grids forCryoelectron Microscopy. 2023. JoVE..."

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== Citation ==

Basanta, Benjamin / Chen, Wenqian / Pride, Daniel E. / Lander, Gabriel C. Fabrication of Monolayer Graphene-Coated Grids forCryoelectron Microscopy. 2023. JoVE (Journal of Visualized Experiments), Vol. 199, p. e65702

== Abstract ==

Cryogenic electron microscopy (cryoEM) has emerged as a powerful technique for
probing the atomic structure of macromolecular complexes. Sample preparation
for cryoEM requires preserving specimens in a thin layer of vitreous ice, typically
suspended within the holes of a fenestrated support film. However, all commonly
used sample preparation approaches for cryoEM studies expose the specimen to
the air-water interface, introducing a strong hydrophobic effect on the specimen
that often results in denaturation, aggregation, and complex dissociation. Further,
preferred hydrophobic interactions between regions of the specimen and the air-water
interface impact the orientations adopted by the macromolecules, resulting in 3D
reconstructions with anisotropic directional resolution.

Adsorption of cryoEM specimens to a monolayer of graphene has been shown to help
mitigate interactions with the air-water interface while minimizing the introduction of
background noise. Graphene supports also offer the benefit of substantially lowering
the required concentration of proteins required for cryoEM imaging. Despite the
advantages of these supports, graphene-coated grids are not widely used by the
cryoEM community due to the prohibitive expense of commercial options and the
challenges associated with large-scale in-house production. This paper describes an
efficient method for preparing batches of cryoEM grids that have nearly full coverage
of monolayer graphene.

== Keywords ==

== Links ==

https://www.jove.com/es/t/65702/author-spotlight-enhancing-cryoem-resolution-using-graphene-coated

== Related software ==

== Related methods ==

== Comments ==

2023Basanta Graphene - Revision history

WikiSysop: Created page with "== Citation == Basanta, Benjamin / Chen, Wenqian / Pride, Daniel E. / Lander, Gabriel C. Fabrication of Monolayer Graphene-Coated Grids forCryoelectron Microscopy. 2023. JoVE..."