WikiSysop: Created page with "== Citation == Fernandez-Gimenez, Estrella / Carazo, J. M. / Sorzano, C. O. S. Local defocus estimation in single particle analysis in cryo-electron microscopy. 2023. J. Structural Biology, Vol. 215, No. 4, p. 108030 == Abstract == Single Particle analysis (SPA) aims to determine the three-dimensional structure of proteins and macromolecular complexes. The current state of the art has allowed us to achieve near-atomic and even atomic resolutions. To obtain high-resolu..."

2024-07-31T05:44:13Z

Created page with "== Citation == Fernandez-Gimenez, Estrella / Carazo, J. M. / Sorzano, C. O. S. Local defocus estimation in single particle analysis in cryo-electron microscopy. 2023. J. Structural Biology, Vol. 215, No. 4, p. 108030 == Abstract == Single Particle analysis (SPA) aims to determine the three-dimensional structure of proteins and macromolecular complexes. The current state of the art has allowed us to achieve near-atomic and even atomic resolutions. To obtain high-resolu..."

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== Citation ==

Fernandez-Gimenez, Estrella / Carazo, J. M. / Sorzano, C. O. S. Local defocus estimation in single particle analysis in cryo-electron microscopy. 2023. J. Structural Biology, Vol. 215, No. 4, p. 108030

== Abstract ==

Single Particle analysis (SPA) aims to determine the three-dimensional structure of proteins and macromolecular
complexes. The current state of the art has allowed us to achieve near-atomic and even atomic resolutions. To
obtain high-resolution structures, a set of well-defined image processing steps is required. A critical one is the
estimation of the Contrast Transfer Function (CTF), which considers the sample defocus and aberrations of the
microscope. Defocus is usually globally estimated; in this case, it is the same for all the particles in each
micrograph. But proteins are ice-embedded at different heights, suggesting that defocus should be measured in a
local (per particle) manner. There are four state-of-the-art programs to estimate local defocus (Gctf, Relion,
CryoSPARC, and Xmipp). In this work, we have compared the results of these software packages to check
whether the resolution improves. We have used the Scipion framework and developed a specific program to
analyze local defocus. The results produced by different programs do not show a clear consensus using the
current test datasets in this study.

== Keywords ==

== Links ==

https://www.sciencedirect.com/science/article/pii/S104784772300093X

== Related software ==

== Related methods ==

== Comments ==

2023Fernandez Local - Revision history