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2023-10-19T07:09:13Z

Created page with "== Citation == Han, Bong-Gyoon / Avila-Sakar, Agustin / Remis, Jonathan / Glaeser, Robert M. Challenges in making ideal cryo-EM samples. 2023. Current Opinion in Structural B..."

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== Citation ==

Han, Bong-Gyoon / Avila-Sakar, Agustin / Remis, Jonathan / Glaeser, Robert M. Challenges in making ideal cryo-EM samples. 2023. Current Opinion in Structural Biology, Vol. 81,
p. 102646

== Abstract ==

Recognizing that interaction with the air–water interface (AWI)
is a major challenge for cryo-EM, we first review current approaches
designed to avoid it. Of these, immobilizing particles
on affinity grids is arguably the most promising. In addition, we
review efforts to gain more reliable control of the sample
thicknesses, not the least important reason being to prevent
immobilized particles from coming in contact with the AWI of
the remaining buffer. It is emphasized that avoiding such a
contact is as important for cryo-ET as for single-particle cryo-
EM. Finally, looking to the future, it is proposed that immobilized
samples might be used to perform time-resolved
biochemical experiments directly on EM grids rather than just
in test tubes or cuvettes.

== Keywords ==

== Links ==

https://www.sciencedirect.com/science/article/pii/S0959440X23001203

== Related software ==

== Related methods ==

== Comments ==

2023Han Sample - Revision history

WikiSysop: Created page with "== Citation == Han, Bong-Gyoon / Avila-Sakar, Agustin / Remis, Jonathan / Glaeser, Robert M. Challenges in making ideal cryo-EM samples. 2023. Current Opinion in Structural B..."