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2023-08-24T05:52:22Z

Created page with "== Citation == Herreros, D. / Kiska, J. / Ram\irez-Aportela, E. / Filipovic, J. / Carazo, J. M. / Sorzano, C. O. S. ZART: A Novel Multiresolution Reconstruction Algorithm wit..."

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== Citation ==

Herreros, D. / Kiska, J. / Ram\irez-Aportela, E. / Filipovic, J. / Carazo, J. M. / Sorzano, C. O. S. ZART: A Novel Multiresolution Reconstruction Algorithm with Motion-blur Correction for Single Particle Analysis. 2023. J. Molecular Biology, Vol. 435, No. 9, p. 168088

== Abstract ==

One of the main purposes of CryoEM Single Particle Analysis is to reconstruct the three-dimensional
structure of a macromolecule thanks to the acquisition of many particle images representing different
poses of the sample. By estimating the orientation of each projected particle, it is possible to recover
the underlying 3D volume by multiple 3D reconstruction methods, usually working either in Fourier or in
real space. However, the reconstruction from the projected images works under the assumption that all
particles in the dataset correspond to the same conformation of the macromolecule. Although this requisite
holds for some macromolecules, it is not true for flexible specimens, leading to motion-induced artefacts
in the reconstructed CryoEM maps. In this work, we introduce a new Algebraic Reconstruction
Technique called ZART, which is able to include continuous flexibility information during the reconstruction
process to improve local resolution and reduce motion blurring. The conformational changes are modelled
through Zernike3D polynomials. Our implementation allows for a multiresolution description of the
macromolecule adapting itself to the local resolution of the reconstructed map. In addition, ZART has also
proven to be a useful algorithm in cases where flexibility is not so dominant, as it improves the overall
aspect of the reconstructed maps by improving their local and global resolution.

== Keywords ==

== Links ==

https://www.sciencedirect.com/science/article/pii/S002228362300150X

== Related software ==

== Related methods ==

== Comments ==

2023Herreros ZART - Revision history

WikiSysop: Created page with "== Citation == Herreros, D. / Kiska, J. / Ram\irez-Aportela, E. / Filipovic, J. / Carazo, J. M. / Sorzano, C. O. S. ZART: A Novel Multiresolution Reconstruction Algorithm wit..."