WikiSysop: Created page with "== Citation == C. J. Langeberg and J. S. Kieft, “A generalizable scaffold-based approach for structure determination of RNAs by cryo-EM,” Nucleic Acids Research, vol. 51, no. 20, pp. e100–e100, 2023. == Abstract == Single-particle cryo-electron microscopy (cryo-EM) can reveal the structures of large and often dynamic molecules, but smaller biomolecules remain challenging targets due to their intrinsic low signal to noise ratio. Methods to resolve small proteins..."

2024-12-27T07:53:00Z

Created page with "== Citation == C. J. Langeberg and J. S. Kieft, “A generalizable scaffold-based approach for structure determination of RNAs by cryo-EM,” Nucleic Acids Research, vol. 51, no. 20, pp. e100–e100, 2023. == Abstract == Single-particle cryo-electron microscopy (cryo-EM) can reveal the structures of large and often dynamic molecules, but smaller biomolecules remain challenging targets due to their intrinsic low signal to noise ratio. Methods to resolve small proteins..."

New page

== Citation ==

C. J. Langeberg and J. S. Kieft, “A generalizable scaffold-based approach for structure determination of RNAs by cryo-EM,” Nucleic Acids Research, vol. 51, no. 20, pp. e100–e100, 2023.

== Abstract ==

Single-particle cryo-electron microscopy (cryo-EM) can reveal the structures of large and often
dynamic molecules, but smaller biomolecules remain challenging targets due to their intrinsic low signal to
noise ratio. Methods to resolve small proteins have been applied but development of similar approaches for
small structured RNA elements have lagged. Here, we present a scaffold-based approach that we used to
recover maps of sub-25 kDa RNA domains to 4.5 - 5.0 Å. While lacking the detail of true high-resolution
maps, these are suitable for model building and preliminary structure determination. We demonstrate this
method faithfully recovers the structure of several RNA elements of known structure, and it promises to be
generalized to other RNAs without disturbing their native fold. This approach may streamline the sample
preparation process and reduce the optimization required for data collection. This first-generation scaffold
approach provides a system for RNA structure determination by cryo-EM and lays the groundwork for
further scaffold optimization to achieve higher resolution.

== Keywords ==

== Links ==

https://academic.oup.com/nar/article/51/20/e100/7288835

== Related software ==

== Related methods ==

== Comments ==

2023Langeberg RNAScaffold - Revision history