WikiSysop: Created page with "== Citation == B. A. Lucas, B. A. Himes, and N. Grigorieff, “Baited reconstruction with 2D template matching for high-resolution structure determination in vitro and in vivo without template bias,” Elife, vol. 12, p. RP90486, 2023. == Abstract == Previously we showed that 2D template matching (2DTM) can be used to localize macromolecular complexes in images recorded by cryogenic electron microscopy (cryo-EM) with high precision, even in the presence of noise and c..."

2025-01-03T08:02:44Z

Created page with "== Citation == B. A. Lucas, B. A. Himes, and N. Grigorieff, “Baited reconstruction with 2D template matching for high-resolution structure determination in vitro and in vivo without template bias,” Elife, vol. 12, p. RP90486, 2023. == Abstract == Previously we showed that 2D template matching (2DTM) can be used to localize macromolecular complexes in images recorded by cryogenic electron microscopy (cryo-EM) with high precision, even in the presence of noise and c..."

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== Citation ==

B. A. Lucas, B. A. Himes, and N. Grigorieff, “Baited reconstruction with 2D template matching for high-resolution structure determination in vitro and in vivo without template bias,” Elife, vol. 12, p. RP90486, 2023.

== Abstract ==

Previously we showed that 2D template matching (2DTM) can be used to localize macromolecular complexes in images recorded by cryogenic electron microscopy (cryo-EM) with high precision, even in the presence of noise and cellular background (Lucas et al., 2021; Lucas et al., 2022). Here, we show that once localized, these particles may be averaged together to generate high-resolution 3D reconstructions. However, regions included in the template may suffer from template bias, leading to inflated resolution estimates and making the interpretation of high-resolution features unreliable. We evaluate conditions that minimize template bias while retaining the benefits of high-precision localization, and we show that molecular features not present in the template can be reconstructed at high resolution from targets found by 2DTM, extending prior work at low-resolution. Moreover, we present a quantitative metric for template bias to aid the interpretation of 3D reconstructions calculated with particles localized using high-resolution templates and fine angular sampling.

== Keywords ==

== Links ==

https://elifesciences.org/articles/90486

== Related software ==

== Related methods ==

== Comments ==

2023Lucas Baited - Revision history