https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2023McMullan_100kV2023McMullan 100kV - Revision history2026-05-24T21:11:37ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=2023McMullan_100kV&diff=4548&oldid=prevWikiSysop: Created page with "== Citation == McMullan, Greg / Naydenova, Katerina / Mihaylov, Daniel / Yamashita, Keitaro / Peet, Mathew J. / Wilson, Hugh / Dickerson, Joshua L. / Chen, Shaoxia / Cannone, Giuseppe / Lee, Yang / others. Structure determination by cryoEM at 100 keV. 2023. Proc. Natl. Acad. Sci. USA , Vol. 120, No. 49, p. e2312905120 == Abstract == Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, spec..."2024-07-11T16:22:53Z<p>Created page with "== Citation == McMullan, Greg / Naydenova, Katerina / Mihaylov, Daniel / Yamashita, Keitaro / Peet, Mathew J. / Wilson, Hugh / Dickerson, Joshua L. / Chen, Shaoxia / Cannone, Giuseppe / Lee, Yang / others. Structure determination by cryoEM at 100 keV. 2023. Proc. Natl. Acad. Sci. USA , Vol. 120, No. 49, p. e2312905120 == Abstract == Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, spec..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
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McMullan, Greg / Naydenova, Katerina / Mihaylov, Daniel / Yamashita, Keitaro / Peet, Mathew J. / Wilson, Hugh / Dickerson, Joshua L. / Chen, Shaoxia / Cannone, Giuseppe / Lee, Yang / others. Structure determination by cryoEM at 100 keV. 2023. Proc. Natl. Acad. Sci. USA , Vol. 120, No. 49, p. e2312905120<br />
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== Abstract ==<br />
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Electron cryomicroscopy can, in principle, determine the structures of most biological<br />
molecules but is currently limited by access, specimen preparation difficulties, and cost.<br />
We describe a purpose-built instrument operating at 100 keV—including advances<br />
in electron optics, detection, and processing—that makes structure determination<br />
fast and simple at a fraction of current costs. The instrument attains its theoretical<br />
performance limits, allowing atomic resolution imaging of gold test specimens and<br />
biological molecular structure determination in hours. We demonstrate its capabilities<br />
by determining the structures of eleven different specimens, ranging in size from<br />
140 kDa to 2 MDa, using a fraction of the data normally required. CryoEM with a<br />
microscope designed specifically for high-efficiency, on-the-spot imaging of biological<br />
molecules will expand structural biology to a wide range of previously intractable<br />
problems.<br />
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== Keywords ==<br />
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== Links ==<br />
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https://www.pnas.org/doi/abs/10.1073/pnas.2312905120<br />
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== Related software ==<br />
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== Related methods ==<br />
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== Comments ==</div>WikiSysop