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	<title>2023Mendez Pipelines - Revision history</title>
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	<updated>2026-06-13T12:18:06Z</updated>
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		<id>https://3demmethods.i2pc.es/index.php?title=2023Mendez_Pipelines&amp;diff=4568&amp;oldid=prev</id>
		<title>WikiSysop: Created page with &quot;== Citation ==  Mendez, Joshua H. / Chua, Eugene Y. D. / Paraan, Mohammadreza / Potter, Clinton S. / Carragher, Bridget. Automated pipelines for rapid evaluation during cryoEM data acquisition 2023. Current Opinion in Structural Biology, Vol. 83, p. 102729  == Abstract ==  Cryo-electron microscopy (cryoEM) has become a popular method for determining high-resolution structures of biomolecules. However, data processing can be time-consuming, particularly for new researcher...&quot;</title>
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		<updated>2024-07-29T05:40:17Z</updated>

		<summary type="html">&lt;p&gt;Created page with &amp;quot;== Citation ==  Mendez, Joshua H. / Chua, Eugene Y. D. / Paraan, Mohammadreza / Potter, Clinton S. / Carragher, Bridget. Automated pipelines for rapid evaluation during cryoEM data acquisition 2023. Current Opinion in Structural Biology, Vol. 83, p. 102729  == Abstract ==  Cryo-electron microscopy (cryoEM) has become a popular method for determining high-resolution structures of biomolecules. However, data processing can be time-consuming, particularly for new researcher...&amp;quot;&lt;/p&gt;
&lt;p&gt;&lt;b&gt;New page&lt;/b&gt;&lt;/p&gt;&lt;div&gt;== Citation ==&lt;br /&gt;
&lt;br /&gt;
Mendez, Joshua H. / Chua, Eugene Y. D. / Paraan, Mohammadreza / Potter, Clinton S. / Carragher, Bridget. Automated pipelines for rapid evaluation during cryoEM data acquisition&lt;br /&gt;
2023. Current Opinion in Structural Biology, Vol. 83, p. 102729&lt;br /&gt;
&lt;br /&gt;
== Abstract ==&lt;br /&gt;
&lt;br /&gt;
Cryo-electron microscopy (cryoEM) has become a popular&lt;br /&gt;
method for determining high-resolution structures of biomolecules.&lt;br /&gt;
However, data processing can be time-consuming,&lt;br /&gt;
particularly for new researchers entering the field. To improve&lt;br /&gt;
data quality and increase data collection efficiency, several&lt;br /&gt;
software packages have been developed for on-the-fly data&lt;br /&gt;
processing with various degrees of automation. These software&lt;br /&gt;
packages allow researchers to perform tasks such as&lt;br /&gt;
motion correction, CTF estimation, 2D classification, and 3D&lt;br /&gt;
reconstruction in real-time, with minimal human input. On-thefly&lt;br /&gt;
data processing can not only improve data collection efficiency&lt;br /&gt;
but also increase the productivity of instrumentation in&lt;br /&gt;
high demand. However, the various software packages available&lt;br /&gt;
differ in their performance, computational requirements,&lt;br /&gt;
and levels of automation. In this review, we describe the minimal&lt;br /&gt;
metrics used to assess data quality during data collection,&lt;br /&gt;
outline the features of an ideal on-the-fly data processing&lt;br /&gt;
software systems, and provide results from using three of&lt;br /&gt;
these systems.&lt;br /&gt;
&lt;br /&gt;
== Keywords ==&lt;br /&gt;
&lt;br /&gt;
== Links ==&lt;br /&gt;
&lt;br /&gt;
https://www.sciencedirect.com/science/article/pii/S0959440X23002038&lt;br /&gt;
&lt;br /&gt;
== Related software ==&lt;br /&gt;
&lt;br /&gt;
== Related methods ==&lt;br /&gt;
&lt;br /&gt;
== Comments ==&lt;/div&gt;</summary>
		<author><name>WikiSysop</name></author>
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