https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2023Schreiber_charge 2026-06-13T13:25:35Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2023Schreiber_charge&diff=4386&oldid=prev 2023-07-25T09:58:31Z

<p>Created page with &quot;== Citation == Schreiber, Makoto Tokoro / Maigné, Alan / Beleggia, Marco / Shibata, Satoshi / Wolf, Matthias. Temporal dynamics of charge buildup in cryo-electron microscopy...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Schreiber, Makoto Tokoro / Maigné, Alan / Beleggia, Marco / Shibata, Satoshi / Wolf, Matthias. Temporal dynamics of charge buildup in cryo-electron microscopy. 2023. J. Structural Biology: X, Vol. 7, p. 100081 <br /> <br /> == Abstract ==<br /> <br /> It is well known that insulating samples can accumulate electric charges from exposure to an electron beam. How<br /> the accumulation of charge affects imaging parameters and sample stability in transmission electron microscopy<br /> is poorly understood. To quantify these effects, it is important to know how the charge is distributed within the<br /> sample and how it builds up over time. In the present study, we determine the spatial distribution and temporal<br /> dynamics of charge accumulation on vitreous ice samples with embedded proteins through a combination of<br /> modeling and Fresnel diffraction experiments. Our data reveal a rapid evolution of the charge state on ice upon<br /> initial exposure to the electron beam accompanied by charge gradients at the interfaces between ice and carbon<br /> films. We demonstrate that ice film movement and charge state variations occur upon electron beam exposure<br /> and are dose-rate dependent. Both affect the image defocus through a combination of sample height changes and<br /> lensing effects. Our results may be used as a guide to improve sample preparation, data collection, and data<br /> processing for imaging of dose-sensitive samples.<br /> <br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.sciencedirect.com/science/article/pii/S2590152422000228<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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