https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2023Seitz_ESPER2023Seitz ESPER - Revision history2026-05-24T22:01:30ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=2023Seitz_ESPER&diff=4462&oldid=prevWikiSysop: Created page with "== Citation == Seitz, Evan / Acosta-Reyes, Francisco / Maji, Suvrajit / Schwander, Peter / Frank, Joachim. Recovery of conformational continuum from single-particle Cryo-EM i..."2023-09-06T12:17:30Z<p>Created page with "== Citation == Seitz, Evan / Acosta-Reyes, Francisco / Maji, Suvrajit / Schwander, Peter / Frank, Joachim. Recovery of conformational continuum from single-particle Cryo-EM i..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
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Seitz, Evan / Acosta-Reyes, Francisco / Maji, Suvrajit / Schwander, Peter / Frank, Joachim. Recovery of conformational continuum from single-particle Cryo-EM images: Optimization of ManifoldEM informed by ground truth. 2023. IEEE Trans. on computational imaging, Vol. 8, p. 462-478 <br />
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== Abstract ==<br />
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This work is based on the manifold-embedding approach<br />
to study biological molecules exhibiting continuous conformational<br />
changes. Previous work established a method—now<br />
termed ManifoldEM—capable of reconstructing 3D movies and<br />
accompanying free-energy landscapes from single-particle cryo-EM images of macromolecules exercising multiple conformational<br />
degrees of freedom. While ManifoldEM has proven its viability<br />
in several experimental studies, critical limitations and uncertainties<br />
have been found throughout its extended development and<br />
use. Guided by insights from studies with cryo-EM ground-truth<br />
data, simulated from atomic structures undergoing conformational<br />
changes, we have built a novel framework, ESPER, able to retrieve<br />
the free-energy landscape and respective 3D Coulomb potential<br />
maps for all states simulated. As shown by a direct comparison<br />
of ground truth vs. recovered maps, and analysis of experimental<br />
data from the 80S ribosome and ryanodine receptor, ESPER offers<br />
substantial improvements relative to the previous work.<br />
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== Keywords ==<br />
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== Links ==<br />
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https://ieeexplore.ieee.org/abstract/document/9773954<br />
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== Related software ==<br />
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== Related methods ==<br />
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== Comments ==</div>WikiSysop