https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2023Venien_Membrane2023Venien Membrane - Revision history2026-06-13T12:16:56ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=2023Venien_Membrane&diff=4497&oldid=prevWikiSysop: Created page with "== Citation == Vénien-Bryan, Catherine / Fernandes, Carlos A. H. Overview of Membrane Protein Sample Preparation for Single-Particle Cryo-Electron Microscopy Analysis. 2023...."2024-01-04T07:41:45Z<p>Created page with "== Citation == Vénien-Bryan, Catherine / Fernandes, Carlos A. H. Overview of Membrane Protein Sample Preparation for Single-Particle Cryo-Electron Microscopy Analysis. 2023...."</p>
<p><b>New page</b></p><div>== Citation ==<br />
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Vénien-Bryan, Catherine / Fernandes, Carlos A. H. Overview of Membrane Protein Sample Preparation for Single-Particle Cryo-Electron Microscopy Analysis. 2023. Intl. J. Molecular Sciences, Vol. 24, No. 19, p. 14785 <br />
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== Abstract ==<br />
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Single-particle cryo-electron microscopy (cryo-EM SPA) has recently emerged as an exceptionally<br />
well-suited technique for determining the structure of membrane proteins (MPs). Indeed, in<br />
recent years, huge increase in the number of MPs solved via cryo-EM SPA at a resolution better than<br />
3.0 Å in the Protein Data Bank (PDB) has been observed. However, sample preparation remains a<br />
significant challenge in the field. Here, we evaluated the MPs solved using cryo-EM SPA deposited<br />
in the PDB in the last two years at a resolution below 3.0 Å. The most critical parameters for sample<br />
preparation are as follows: (i) the surfactant used for protein extraction from the membrane, (ii) the<br />
surfactant, amphiphiles, nanodiscs or other molecules present in the vitrification step, (iii) the vitrification<br />
method employed, and (iv) the type of grids used. The aim is not to provide a definitive<br />
answer on the optimal sample conditions for cryo-EM SPA of MPs but rather assess the current trends<br />
in the MP structural biology community towards obtaining high-resolution cryo-EM structures.<br />
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== Keywords ==<br />
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== Links ==<br />
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https://www.mdpi.com/1422-0067/24/19/14785<br />
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== Related software ==<br />
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== Related methods ==<br />
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== Comments ==</div>WikiSysop