https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2023Zhao_Review 2026-05-24T20:20:48Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2023Zhao_Review&diff=4524&oldid=prev 2024-01-10T07:51:56Z

<p>Created page with &quot;== Citation == Zhao, Cuicui / Lu, Da / Zhao, Qian / Ren, Chongjiao / Zhang, Huangtao / Zhai, Jiaqi / Gou, Jiaxin / Zhu, Shilin / Zhang, Yaqi / Gong, Xinqi. Computational meth...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Zhao, Cuicui / Lu, Da / Zhao, Qian / Ren, Chongjiao / Zhang, Huangtao / Zhai, Jiaqi / Gou, Jiaxin / Zhu, Shilin / Zhang, Yaqi / Gong, Xinqi. Computational methods for in situ structural studies with cryogenic electron tomography. 2023. Frontiers in Cellular and Infection Microbiology, Vol. 13, 1135013<br /> <br /> == Abstract ==<br /> <br /> Cryo-electron tomography (cryo-ET) plays a critical role in imaging<br /> microorganisms in situ in terms of further analyzing the working mechanisms<br /> of viruses and drug exploitation, among others. A data processing workflow for<br /> cryo-ET has been developed to reconstruct three-dimensional density maps and<br /> further build atomic models from a tilt series of two-dimensional projections.<br /> Low signal-to-noise ratio (SNR) and missing wedge are two major factors that<br /> make the reconstruction procedure challenging. Because only few near-atomic<br /> resolution structures have been reconstructed in cryo-ET, there is still much<br /> room to design new approaches to improve universal reconstruction resolutions.<br /> This review summarizes classical mathematical models and deep learning<br /> methods among general reconstruction steps. Moreover, we also discuss<br /> current limitations and prospects. This review can provide software and<br /> methods for each step of the entire procedure from tilt series by cryo-ET to<br /> 3D atomic structures. In addition, it can also help more experts in various fields<br /> comprehend a recent research trend in cryo-ET. Furthermore, we hope that<br /> more researchers can collaborate in developing computational methods and<br /> mathematical models for high-resolution three-dimensional structures from<br /> cryo-ET datasets.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.frontiersin.org/articles/10.3389/fcimb.2023.1135013/full<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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