WikiSysop: Created page with "== Citation == Zhu, Jianying / Zhang, Qi / Zhang, Hui / Shi, Zuoqiang / Hu, Mingxu / Bao, Chenglong. A minority of final stacks yields superior amplitude in single-particle cryo-EM. 2023. Nature Communications, Vol. 14, No. 1, p. 7822 == Abstract == Cryogenic electron microscopy (cryo-EM) is widely used to determine nearatomic resolution structures of biological macromolecules. Due to the low signal-to-noise ratio, cryo-EM relies on averaging many images. However, a c..."

2024-07-30T06:01:11Z

Created page with "== Citation == Zhu, Jianying / Zhang, Qi / Zhang, Hui / Shi, Zuoqiang / Hu, Mingxu / Bao, Chenglong. A minority of final stacks yields superior amplitude in single-particle cryo-EM. 2023. Nature Communications, Vol. 14, No. 1, p. 7822 == Abstract == Cryogenic electron microscopy (cryo-EM) is widely used to determine nearatomic resolution structures of biological macromolecules. Due to the low signal-to-noise ratio, cryo-EM relies on averaging many images. However, a c..."

New page

== Citation ==

Zhu, Jianying / Zhang, Qi / Zhang, Hui / Shi, Zuoqiang / Hu, Mingxu / Bao, Chenglong. A minority of final stacks yields superior amplitude in single-particle cryo-EM. 2023. Nature Communications, Vol. 14, No. 1, p. 7822

== Abstract ==

Cryogenic electron microscopy (cryo-EM) is widely used to determine nearatomic
resolution structures of biological macromolecules. Due to the low
signal-to-noise ratio, cryo-EM relies on averaging many images. However, a
crucial question in the field of cryo-EM remains unanswered: how close can we
get to the minimumnumber of particles required to reach a specific resolution
in practice? The absence of an answer to this question has impeded progress in
understanding sample behavior and the performance of sample preparation
methods. To address this issue, we develop an iterative particle sorting and/or
sieving method called CryoSieve. Extensive experiments demonstrate that
CryoSieve outperforms other cryo-EM particle sorting algorithms, revealing
that most particles are unnecessary in final stacks. The minority of particles
remaining in the final stacks yield superior high-resolution amplitude in
reconstructed density maps. For some datasets, the size of the finest subset
approaches the theoretical limit.

== Keywords ==

== Links ==

https://www.nature.com/articles/s41467-023-43555-x

== Related software ==

== Related methods ==

== Comments ==

2023Zhu CryoSieve - Revision history