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	<title>2024Bobe Calibration - Revision history</title>
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	<updated>2026-05-24T22:01:27Z</updated>
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	<entry>
		<id>https://3demmethods.i2pc.es/index.php?title=2024Bobe_Calibration&amp;diff=5056&amp;oldid=prev</id>
		<title>WikiSysop: Created page with &quot;== Citation ==  Bobe, D., Kopylov, M., Miller, J., Owji, A.P. and Eng, E.T. 2024. Multi-species cryoEM calibration and workflow verification standard. Structural Biology and Crystallization Communications. 80, 11 (2024), 320–327.  == Abstract ==  Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established biophysical and analytical tech...&quot;</title>
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		<updated>2025-08-29T08:39:13Z</updated>

		<summary type="html">&lt;p&gt;Created page with &amp;quot;== Citation ==  Bobe, D., Kopylov, M., Miller, J., Owji, A.P. and Eng, E.T. 2024. Multi-species cryoEM calibration and workflow verification standard. Structural Biology and Crystallization Communications. 80, 11 (2024), 320–327.  == Abstract ==  Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established biophysical and analytical tech...&amp;quot;&lt;/p&gt;
&lt;p&gt;&lt;b&gt;New page&lt;/b&gt;&lt;/p&gt;&lt;div&gt;== Citation ==&lt;br /&gt;
&lt;br /&gt;
Bobe, D., Kopylov, M., Miller, J., Owji, A.P. and Eng, E.T. 2024. Multi-species cryoEM calibration and workflow verification standard. Structural Biology and Crystallization Communications. 80, 11 (2024), 320–327.&lt;br /&gt;
&lt;br /&gt;
== Abstract ==&lt;br /&gt;
&lt;br /&gt;
Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology&lt;br /&gt;
modality that has been successful in revealing molecular details of biological&lt;br /&gt;
systems. However, unlike established biophysical and analytical techniques with&lt;br /&gt;
calibration standards, cryoEM has lacked comprehensive biological test samples.&lt;br /&gt;
We introduce a cryoEM calibration sample that is a mixture of compatible&lt;br /&gt;
macromolecules that can be used not only for resolution optimization but also&lt;br /&gt;
provides multiple reference points for evaluating instrument performance, data&lt;br /&gt;
quality, and image processing workflows in a single experiment. This combined test&lt;br /&gt;
specimen provides researchers a reference point for validating their cryoEM pipeline,&lt;br /&gt;
benchmarking their methodologies, and testing new algorithms.&lt;br /&gt;
&lt;br /&gt;
== Keywords ==&lt;br /&gt;
&lt;br /&gt;
== Links ==&lt;br /&gt;
&lt;br /&gt;
https://journals.iucr.org/f/issues/2024/11/00/rf5044/index.html&lt;br /&gt;
&lt;br /&gt;
== Related software ==&lt;br /&gt;
&lt;br /&gt;
== Related methods ==&lt;br /&gt;
&lt;br /&gt;
== Comments ==&lt;/div&gt;</summary>
		<author><name>WikiSysop</name></author>
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