https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2024Burt_Relion5 2026-05-24T18:05:25Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2024Burt_Relion5&diff=4729&oldid=prev 2024-08-21T11:56:33Z

<p>Created page with &quot;== Citation == Burt, Alister / Toader, Bogdan / Warshamanage, Rangana / von Kügelgen, Andriko / Pyle, Euan / Zivanov, Jasenko / Kimanius, Dari / Bharat, Tanmay A. M. / Scheres, Sjors H. W. An image processing pipeline for electron cryo-tomography in RELION-5. 2024. FEBS Open Bio == Abstract == Electron tomography of frozen, hydrated samples allows structure determination of macromolecular complexes that are embedded in complex environments. Provided that the target c...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Burt, Alister / Toader, Bogdan / Warshamanage, Rangana / von Kügelgen, Andriko / Pyle, Euan / Zivanov, Jasenko / Kimanius, Dari / Bharat, Tanmay A. M. / Scheres, Sjors H. W. An image processing pipeline for electron cryo-tomography in RELION-5. 2024. FEBS Open Bio<br /> <br /> == Abstract ==<br /> <br /> Electron tomography of frozen, hydrated samples allows structure determination<br /> of macromolecular complexes that are embedded in complex environments.<br /> Provided that the target complexes may be localised in noisy,<br /> three-dimensional tomographic reconstructions, averaging images of multiple<br /> instances of these molecules can lead to structures with sufficient resolution<br /> for de novo atomic modelling. Although many research groups have<br /> contributed image processing tools for these tasks, a lack of standardisation<br /> and interoperability represents a barrier for newcomers to the field.<br /> Here, we present an image processing pipeline for electron tomography<br /> data in RELION-5, with functionality ranging from the import of unprocessed<br /> movies to the automated building of atomic models in the final<br /> maps. Our explicit definition of metadata items that describe the steps of<br /> our pipeline has been designed for interoperability with other software<br /> tools and provides a framework for further standardisation.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://febs.onlinelibrary.wiley.com/doi/full/10.1002/2211-5463.13873<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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