WikiSysop: Created page with "== Citation == C. J. Cameron, S. J. Seager, F. J. Sigworth, H. D. Tagare, and M. B. Gerstein, “REliable PIcking by Consensus (REPIC): a consensus methodology for harnessing multiple cryo-EM particle pickers,” Communications Biology, vol. 7, no. 1, p. 1421, 2024. == Abstract == Cryo-EM particle identification from micrographs (“picking”) is challenging due to the low signal-to-noise ratio and lack of ground truth for particle locations. State-of-the-art computa..."

2024-11-08T10:31:54Z

Created page with "== Citation == C. J. Cameron, S. J. Seager, F. J. Sigworth, H. D. Tagare, and M. B. Gerstein, “REliable PIcking by Consensus (REPIC): a consensus methodology for harnessing multiple cryo-EM particle pickers,” Communications Biology, vol. 7, no. 1, p. 1421, 2024. == Abstract == Cryo-EM particle identification from micrographs (“picking”) is challenging due to the low signal-to-noise ratio and lack of ground truth for particle locations. State-of-the-art computa..."

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== Citation ==

C. J. Cameron, S. J. Seager, F. J. Sigworth, H. D. Tagare, and M. B. Gerstein, “REliable PIcking by Consensus (REPIC): a consensus methodology for harnessing multiple cryo-EM particle pickers,” Communications Biology, vol. 7, no. 1, p. 1421, 2024.

== Abstract ==

Cryo-EM particle identification from micrographs (“picking”) is challenging due to the low
signal-to-noise ratio and lack of ground truth for particle locations. State-of-the-art computational
algorithms (“pickers”) identify different particle sets, complicating the selection of the best-suited
picker for a protein of interest. Here, we present REliable PIcking by Consensus (REPIC), a
computational approach to identifying particles common to the output of multiple pickers. We frame
consensus particle picking as a graph problem, which REPIC solves using integer linear programming.
REPIC picks high-quality particles even when the best picker is not known a priori or a protein is
difficult-to-pick (e.g., NOMPC ion channel). Reconstructions using consensus particles without
particle filtering achieve resolutions comparable to those fromparticles picked by experts. Our results
show that REPIC requires minimal (often no) manual intervention, and considerably reduces the
burden on cryo-EM users for picker selection and particle picking. Availability: https://github.com/
ccameron/REPIC.

== Keywords ==

== Links ==

https://www.nature.com/articles/s42003-024-07045-0

== Related software ==

== Related methods ==

== Comments ==

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