https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2024Chen_Focused 2026-05-24T19:36:16Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2024Chen_Focused&diff=4541&oldid=prev 2024-01-19T07:45:08Z

<p>Created page with &quot;== Citation == Chen, Muyuan / Schmid, Michael F. / Chiu, Wah. Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models. 2024....&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Chen, Muyuan / Schmid, Michael F. / Chiu, Wah. Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models. 2024. Nature Methods, Vol. 21, p. 37-40 <br /> <br /> == Abstract ==<br /> <br /> Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we developed a refinement protocol based on Gaussian mixture models that integrates particle orientation and conformation estimation and improves the alignment for flexible domains of protein structures. We demonstrated this protocol on multiple datasets, resulting in improved resolution and resolvability, locally and globally, by visual and quantitative measures.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.nature.com/articles/s41592-023-02082-9<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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