WikiSysop: Created page with "== Citation == Cheng, Anchi / Yu, Yue. Recent advances in data collection for Cryo-EM methods. 2024. Current Opinion in Structural Biology, Vol. 86, p. 102795 == Abstract == Methods of transmission electron microscopy (TEM) are typically used to resolve structures of vitrified biological specimens using both single particle analysis (SPA) and tomographic methods and use both conventional as well as scanning transmission modes of data collection. Automation of data col..."

2024-08-14T06:19:34Z

Created page with "== Citation == Cheng, Anchi / Yu, Yue. Recent advances in data collection for Cryo-EM methods. 2024. Current Opinion in Structural Biology, Vol. 86, p. 102795 == Abstract == Methods of transmission electron microscopy (TEM) are typically used to resolve structures of vitrified biological specimens using both single particle analysis (SPA) and tomographic methods and use both conventional as well as scanning transmission modes of data collection. Automation of data col..."

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== Citation ==

Cheng, Anchi / Yu, Yue. Recent advances in data collection for Cryo-EM methods. 2024. Current Opinion in Structural Biology, Vol. 86, p. 102795

== Abstract ==

Methods of transmission electron microscopy (TEM) are typically
used to resolve structures of vitrified biological specimens
using both single particle analysis (SPA) and tomographic
methods and use both conventional as well as scanning
transmission modes of data collection. Automation of data
collection for each method has been developed to different
levels of convenience for the users. Automation of methods
using the conventional TEM mode has progressed the furthest.
Beam-image shift strategies first used in data collection for
SPA were shown to be equally valuable for cryo-electron tomography
(cryo-ET). Machine learning methods have been
applied for target selection and for planning optimal paths of
data collection for SPA. These methods also enabled automated
screening. Apertures matching the square shape of
cameras have been recently described. Some progress has
also been made in the automation of cryo applications of
scanning TEM, promising an increase of throughput and potential
for further improvement.

== Keywords ==

== Links ==

https://www.sciencedirect.com/science/article/pii/S0959440X24000228

== Related software ==

== Related methods ==

== Comments ==

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